| Abstract |
In 2010 to 2011, “Gondalbi” (Ligularia stenocephala) leaves and roots with abnormal characteristics, such as leaf spot and blight, necrosis, powdery mildew, chlorosis, and root rot symptoms were collected in field of Yanggu, Gangwon Province. Similar symptoms have been reported by local farmers since 2008, and they required the identity of those associated pathogens to build up environment-friendly control strategies. Symptoms were primarily divided to black spotted leaf blight, anthracnose, brown leaf spot, leaf blight, powdery mildew, vein necrosis, chlorosis, and root rot based on their typical characteristics, which are the most frequent lesions produced in leaves and roots. In the field, the productions of black spotted leaf blight, anthracnose, brown leaf spot, and leaf blight diseases were highest with daily mean diurnal temperature regimes from 10 to 20℃ and >60% relative humidity; root rot and chlorosis severities was highest in the range of 5 to 10℃, whereas >20℃ of diurnal temperature at the late of harvesting season appeared to be related to vein necrosis and powdery mildew. Twelve fungal stains were isolated from disease samples using potato dextrose agar (PDA) supplemented with kanamycin. Phylogenetic analysis on the basis of partial sequences of the 18S rDNA and ITS region of twelve isolates and reference fungi available in the GenBank, the isolates from black spotted leaf blight, anthracnose, brown leaf spot, and root rot diseases were similarly clustered into Phoma glomerata, Colletotrichum spp., Alternaria brassicae, and Rhizoctonia spp., respectively. To our knowledge, this is the first documented report of the presence of several fungal pathogens and these occurrences increase a significant economic impact to “Gondalbi” at Yanggu country.
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5 조세열1.pdf
