| Article Info |
제24권 3호 강원 농업생명환경연구 Vol.24, No.3, pp.1~8, 2012
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| Title |
In-Vivo Chromosome Doubling with Colchicine in Haploid Plants of Minipaprika (Capsicum annuum L.)
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| Author |
Luitel, B. P. and W. H. Kang
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| Institutions |
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| Abstract |
Haploids production in minipaprika through in-vitro androgenesis is a rapid and simple biotechnological method. This study was conducted for doubling the chromosome through colchicine treatment in anther-derived haploid plants of minipaprika in March, 2012. Eleven haploid plants were treated with colchicine (0.5 %, w/v) through in-vivo method. The axillary branches were removed and colchicine was applied with lanolin paste at the removal portion and shoots were decapitated. Ploidy level was further determined morphologically and cytologically at the colchicine treated plants. Out of eleven plants treated, only seven (63.6%) plants were regenerated with forming the new shoots. Flow cytometry analysis showed that five (71.4%) plants were identified as chimeric haploids and remaining two (28.6%) plants identified as doubled haploids. Chimeric haploids showed lower plant height, smaller leaf and flower bud size with forming the seedless fruit whereas doubled haploids showed bigger leaf size with larger fruit size containing fertile seeds. These doubled haploids can be used in the development of inbred lines for the minipaprika breeding and remaining chimeric haploids is further recommended to decapitate for regenerating the new diploid shoots and producing dihaploid lines.
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| Keyword |
Androgenesis, Colchicine, Doubled haploid, Inbred lines, In-vitro, In-vivo.
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 1-강원희(최종)_1.pdf
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