| Article Info | 제28권 3호 강원 농업생명환경연구 Vol.28, No.3, pp.32-37, 2016 |
| Title | Constitutive Expression of Xylose Reductase Gene Enhances Xylitol Production in Pichia stipitis |
| Author | Kwon, H. J.1 , E. H. Park1 and M. D. Kim1* |
| Institutions | 1Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, Korea |
| Abstract |
To enhance the xylitol production in xylose-fermenting yeast Pichia stipitis, an expression vector was constructed to constitutively express the xylose reductase gene in P. stipitis. While the control P. stipitis strain produced 40.6 ± 0.9 g ethanol from 100 g xylose in aerobic shake flasks cultivation, without forming xylitol, P. stipitis MBY1381, which constitutively expresses the xylose reductase gene by the vector, produced 34.9 ± 0.9 g xylitol utilizing 40 g xylose, corresponding to an approximate yield of 87%. |
| Keyword | Expression, Pichia stipitis, Vector, Xylitol, Xylose reductase. |
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