Article Info 제28권 3호 강원 농업생명환경연구 Vol.28, No.3, pp.38-45, 2016
Title Enhancement of Antiobesogenic Efficacy of Inonotus obliquus by Lactic Acid Bacteria Fermentation
Author Chung, B. H.1 , E. H. Park2 , M. O. Lee1 and M. D. Kim2*
Institutions 1Research and Development Institute, Haesong KNS, Chuncheon 24232, Korea
2Department of Food Science and Biotechnology, Kangwon National University, Chuncheon 24341, Korea 
Abstract The anti-obesity effects of mixed fermented extracts from lactic acid bacteria and Inonotus obliquus were investigated
using differentiation of 3T3-L1 pre-adipocytes to adipocytes as a read out. Adipocyte differentiation was assessed on
the basis of lipid accumulation (measured with Oil Red O assay) and RT-PCR analysis. Compared to the control,
treatment of differentiating adipocytes with an extract from Lactobacillus lactis, Lactobacillus fermentum, and I.
obliquus significantly inhibited differentiation (by 35–50%), as assessed by measuring fat accumulation. In the
presence of an adipogenic cocktail (isobutylmethylxanthine, dexamethasone, and insulin), an extract from L.
fermentum and I. obliquus significantly decreased the expression of the obesity-related genes, peroxisome proliferator –activated receptor-γ (PPARγ) and adipocyte fatty acid–binding protein (aP2), relative to the expression level of
NAC (negative control). These results indicate that the fermented extract from Lac. fermentum and I. obliquusis the
most plausible candidate for preventing obesity.
Keyword Antiobesogenic efficacy, Fermentation, Inonotus obliquus, Lactic acid bacteria, Lipid accumulation.
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