| Article Info |
제29권 3호 강원 농업생명환경연구 Vol.29, No.3, pp.193-201, 2017
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| Title |
Evaluation of Anti-obesity Effect of Roasted Inonotus obliquus in 3T3-L1 Cells
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| Author |
Kim, N. E., M. O. Lee, M. H. Jang and B. H. Chung*
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| Institutions |
Research and Development Institute, Haesong KNS, Chuncheon 24232, Korea
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| Abstract |
The anti-obesity effects of extracts of Inonotus obliquus and roasted I. obliquus were investigated through the measurement of the differentiation of 3T3-L1 pre-adipocytes to adipocytes. Cytotoxic activity was determined by MTT assays on 3T3-L1 pre-adipocyte. Adipocyte differentiation was assessed through the evaluation of lipid accumulation (by using Oil Red O staining) and the expression of adipogenic proteins (by using western blotting). Compared with the control, the treatment of differentiating adipocytes with extracts of I. obliquus , and roasted I. obliquus significantly inhibited differentiation. In the presence of an adipogenic cocktail (isobutyl-methylxanthine, dexamethasone, and insulin), I. obliquus and roasted I. obliquus extracts significantly decreased the expression of adipogenic proteins, peroxisome proliferator-activated receptor-γ (PPARγ), and CCAAT/enhancer binding protein alpha (C/EBP-α), relative to the expression level of N-acetyl-L-cystein (NAC, negative control). These results indicated that the extracts of I. obliquus and roasted I. obliquus were potential candidates for the prevention obesity.
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| Keyword |
Anti-obesity, Inonotus obliquus, Lipid accumulation, Roasting
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 05_(193-201)J4_201700035 정병희.pdf
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