2School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Korea
3Centre for Food Technology, Department of Biotechnology, Anna University, Chennai 600025, India
02_(12-30)J4_201700022 오덕환.pdf
| Article Info | 제30권 1호 강원 농업생명환경연구 Vol.30, No.1, pp.12-30, 2018 |
| Title | Antihypertensive Effect of Peptides from Sesame, Almond, and Pumpkin Seeds: In-silico and In-vivo Evaluation |
| Author | Chelliah, R.1,3, S. R. Ramakrishnan2,3, U. Antony3 , S. H. Kim1 , I. Khan1 , C. N. Tango1 , P. N. Kounkeu1 , S. Wei1 , M. S. Hussain1 , E. B. M. Daliri1 , R. Momna1 , M. Y. Kwon1 , E. H. Lee1 , H. Y. Jo1 , S. B. Hwang1 , E. J. Park1 , H. J. Kim1 and D. H. Oh1* |
| Institutions |
1Department of Food Science and Biotechnology, College of Agriculture and Life Science, Kangwon National University, Chuncheon 24341, Korea 2School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Korea 3Centre for Food Technology, Department of Biotechnology, Anna University, Chennai 600025, India |
| Abstract | Plant proteins are important functional ingredients in many processed food products. In particular, globular proteins from various sources play an important role in many food products. In the current study, 11S globulin protein from white sesame seeds, amandin protein from almond seeds, and cucurbitin protein from pumpkin seeds were extracted by means of ammonium sulfate precipitation and purified by anion-exchange chromatography on a DEAE-Sephadex column (20 × 30 cm). Amandin protein of almond and cucurbitin protein of pumpkin seeds both belong to the 11S globulin family. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of protein samples treated with reducing agents revealed that the isolated 11S globulin from white sesame seeds was composed of an acidic (30－33 kDa) and basic (20－24 kDa) polypeptide, amandin from almond seeds was composed of an acidic (40－42 kDa) and basic (15－20 kDa) polypeptide, and cucurbitin from pumpkin seeds was composed of an acidic (35－42 kDa) and basic (20－25 kDa) polypeptide. The alpha amylase-inhibiting activities of the three proteins was determined. Cucurbitin of pumpkin seeds had a greater alpha amylase inhibitory effect (－86%) than 11S globulin of sesame (82.6%) and amandin protein of almond seeds (76%). The anti-hypertensive effect of the three proteins was evaluated by a chrioallantoin membrane assay in chick embryos, which revealed that cucurbitin protein showed higher vasodilatation activity than the other two proteins. |
| Keyword | Alpha amylase, Amandin, Antihypertensive, Cucurbitin, Globular proteins |
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02_(12-30)J4_201700022 오덕환.pdf
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