| Article Info | 제31권 3호 강원 농업생명환경연구 Vol.31, No.3, pp.121-127, 2019 |
| Title | Comparison of Genome Sequence and Biological Properties of Broad bean wilt virus 2 Isolates from Gynura procumbens |
| Author | Choi, S. A.1 , T. S. Park2 , J. S. Park2 , D. J. Min1 and J. S. Hong3* |
| Institutions |
1Master’s Course, Division of Bioresource Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, Korea 2Ph.D. Course, Division of Bioresource Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, Korea 3Professor, Division of Bioresource Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, Korea |
| Abstract | Gynura procumbens (Myeongwolcho or longevity spinach) showing virus-like mosaic and yellowing symptoms was collected in Chuncheon. To identify the virus from G. procumbens, RT-PCR analysis was performed using several genus-specific primers sets. BBWV2-Gyp was identified from the amplified target product using Fabavirus genus-specific primers. BBWV2- Gyp had a similar host range pattern to BBWV2–SN that has already reported in G. procumbens. BBWV2-Gyp induced systemic mosaic and BBWV2-SN induced systemic necrotic spots on Phaseolus angularis. To investigate the sequence specificity, BBWV2-Gyp RNA2 was sequenced and compared with other BBWV2 isolates. Sequence homologies and phylogenetic analyses clearly indicated that BBWV2-Gyp had the closest relationship to BBWV2-SN. Also, BBWV2- Gyp had 98.5%, 98.8% and 98.5% homologies with movement protein (MP), large coat protein (LCP) and small coat protein (SCP) segments, respectively, of BBWV2-SN at amino acid level. Although two BBWV2 isolates from G. procumbens have high nucleotide sequence similarity (99%), they have distinct biological properties. |
| Keyword | Biological properties, Broad bean wilt virus 2, Gynura procumbens , Host range |
 06_J4_201900019_F1_홍진성(121-127)최종본.pdf
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